Suprachiasmatic astrocytes as an interphase for immune-circadian signalling.

ML LEONE; L MARPEGAN,; T BEKINSCHTEIN,; M COSTAS; DA GOLOMBEK

JOURNAL OF NEUROSCIENCE RESEARCH

Año: 2006 vol. 84 p. 1521 - 1527

0360-4012

The hypothalamic suprachiasmatic nuclei (SCN), the site of a mammalian circadian clock, exhibit a dense immunoreactivity for glial fibrillary acidic protein (GFAP), a specific marker for astrocytes. Although there is evidence of a circadian variation in GFAP-IR in the hamster SCN and of the participation of glial cells in input and output mechanisms of the clock, the role of these cells within the circadian system is not clearly understood. The fact that astroglia can express and respond to cytokines suggests that they could work as mediators of immune signals to the circadian system. In the present study, we have found a daily variation of GFAP-IR in the mouse SCN, peaking during the light phase. In addition, we have identified GFAP and nuclear factor-jB (NF-jB) in glial cells within the SCN and in primary cultures of the mouse SCN. Moreover, SCN glia cultures were transfected with an NF-jB/luc construct whose transcriptional activity was increased with lipopolysaccharide 2 lg/ml, tumor necrosis factor-a 20 ng/ml, or interleukin-1a 100 ng/ ml, after 12 hr of stimulation. These results suggest that the glial cells of the SCN can mediate input signals to the mouse circadian system coming from the immune system via NF-jB signaling. jB (NF-jB) in glial cells within the SCN and in primary cultures of the mouse SCN. Moreover, SCN glia cultures were transfected with an NF-jB/luc construct whose transcriptional activity was increased with lipopolysaccharide 2 lg/ml, tumor necrosis factor-a 20 ng/ml, or interleukin-1a 100 ng/ ml, after 12 hr of stimulation. These results suggest that the glial cells of the SCN can mediate input signals to the mouse circadian system coming from the immune system via NF-jB signaling. jB/luc construct whose transcriptional activity was increased with lipopolysaccharide 2 lg/ml, tumor necrosis factor-a 20 ng/ml, or interleukin-1a 100 ng/ ml, after 12 hr of stimulation. These results suggest that the glial cells of the SCN can mediate input signals to the mouse circadian system coming from the immune system via NF-jB signaling. lg/ml, tumor necrosis factor-a 20 ng/ml, or interleukin-1a 100 ng/ ml, after 12 hr of stimulation. These results suggest that the glial cells of the SCN can mediate input signals to the mouse circadian system coming from the immune system via NF-jB signaling. a 20 ng/ml, or interleukin-1a 100 ng/ ml, after 12 hr of stimulation. These results suggest that the glial cells of the SCN can mediate input signals to the mouse circadian system coming from the immune system via NF-jB signaling. jB signaling.