ACTIVATION OF PPAR ALFA AND PPAR BETA/DELTA REGULATES SERTOLI CELL METABOLISM

REGUEIRA MARIANA; RIERA MARÍA FERNANDA; GALARDO MARÍA NOEL; PELLIZZARI ELIANA HERMINIA; CIGORRAGA SELVA BEATRIZ; MERONI SILVINA BEATRIZ

MOLECULAR AND CELLULAR ENDOCRINOLOGY.

ELSEVIER IRELAND LTD

Lugar: Amsterdam; Año: 2014 vol. 38 p. 271 - 281

0303-7207

The purpose of this study was to evaluate the existence of a possible simultaneous regulation of fatty acid (FA) metabolism and lactate production by PPAR A and PPAR B/D activation in Sertoli cells (SC). SC cultures obtained from 20-day-old rats were incubated with WY14643 or GW0742, pharmacological activators of PPAR A and PPAR B/D respectively. The fatty acid transporter CD36, carnitine palmitoyltransferase 1, long- and medium-chain 3-hydroxyacyl-CoA dehydrogenases mRNA levels were analyzed. An increase in the above-mentioned genes in response to activation of both nuclear receptors was observed. Additionally, PPAR B/D activation increased lactate production as a consequence of increased pyruvate availability by inhibiting the Pyruvate Dehydrogenase Complex. Altogether, these results suggest that in SC, PPAR A activation participates in the regulation of FA metabolism. On the other hand, PPAR B/D activation regulates FA metabolism and lactate production ensuring simultaneously the energetic metabolism for SC and germ cells.