STRESS EFFECT ON THE EXPRESION OF COK5 AND P35 PROTEINS IN BRAIN AREAS OF THE AVERSIVE CIRCUIT

BIGNANTE EA; BUSSOLINO D; PAGLINI MG; MOLINA VA

Pinamar, BsAs

Congreso; XX Sociedad Argentina de Neuroquimica (SAN); 2005

XX Sociedad Argentina de Neuroquimica (SAN)

STRESS EFFECT ON THE EXPRESION OF COK5 AND P35 PROTEINS IN BRAIN AREAS OF THE AVERSIVE CIRCUIT Birmante EA´, Busso/ino D´, Paglini MGJ, Molina VA / Departamento de Farmacología. Facultad de Ciencias Químicas. Universidad Nacional de Córdoba. ]Instituto Mercedes y Martín Ferreyra. E-mail: abignante@fcq.unc.edu.ar Methods: Males Wistar rats (290-320 grs) were restraincd during l hour or 30 minutes and immediately or 30 minutes after stress sacrificed for immunochemistry assay. Another group of animals, were cannulatcd in the lateral septum or the baso lateral amygdala for the infusion of olomucine (40 nmlul) a Cdk5 inhibitor; or isolomucine (40 nmlul) an inactive analogous of olomucine; or vehicule 15 min before restraint. One day later, anxiety was measured in the elevated plus maze (EPM). Results: A l h stress session increased the levels ofCdk5 and p35 in cingulate cortex (Cg), cortical amygdala (ACo) and dorsal lateral septum (LSD) whercasJO minutes ofrestraint increased the levels of p35 in basal amygdala (BLA), dentate gyrus (DO) and the dorsomedial portion ofperiaqueductal grey substance (PAODM). Also, restraint reduced the percentage of time spent in open arms of EPM and this decrease was normalized by olomucine, but not after isolomucine or vehicule, administered ei ther in the lateral septum or in the basolateral amygdala. Conclusions: Stress increases the expression of Cdk5 y p35 in brain arcas involved in the processing of emotional information. This enhancement. at least in lateral septum and basolateral amygdala. could be functionally associated with thc gencration of emotional sensitization. Methods: Males Wistar rats (290-320 grs) were restraincd during l hour or 30 minutes and immediately or 30 minutes after stress sacrificed for immunochemistry assay. Another group of animals, were cannulatcd in the lateral septum or the baso lateral amygdala for the infusion of olomucine (40 nmlul) a Cdk5 inhibitor; or isolomucine (40 nmlul) an inactive analogous of olomucine; or vehicule 15 min before restraint. One day later, anxiety was measured in the elevated plus maze (EPM). Results: A l h stress session increased the levels ofCdk5 and p35 in cingulate cortex (Cg), cortical amygdala (ACo) and dorsal lateral septum (LSD) whercasJO minutes ofrestraint increased the levels of p35 in basal amygdala (BLA), dentate gyrus (DO) and the dorsomedial portion ofperiaqueductal grey substance (PAODM). Also, restraint reduced the percentage of time spent in open arms of EPM and this decrease was normalized by olomucine, but not after isolomucine or vehicule, administered ei ther in the lateral septum or in the basolateral amygdala. Conclusions: Stress increases the expression of Cdk5 y p35 in brain arcas involved in the processing of emotional information. This enhancement. at least in lateral septum and basolateral amygdala. could be functionally associated with thc gencration of emotional sensitization. Methods: Males Wistar rats (290-320 grs) were restraincd during l hour or 30 minutes and immediately or 30 minutes after stress sacrificed for immunochemistry assay. Another group of animals, were cannulatcd in the lateral septum or the baso lateral amygdala for the infusion of olomucine (40 nmlul) a Cdk5 inhibitor; or isolomucine (40 nmlul) an inactive analogous of olomucine; or vehicule 15 min before restraint. One day later, anxiety was measured in the elevated plus maze (EPM). Results: A l h stress session increased the levels ofCdk5 and p35 in cingulate cortex (Cg), cortical amygdala (ACo) and dorsal lateral septum (LSD) whercasJO minutes ofrestraint increased the levels of p35 in basal amygdala (BLA), dentate gyrus (DO) and the dorsomedial portion ofperiaqueductal grey substance (PAODM). Also, restraint reduced the percentage of time spent in open arms of EPM and this decrease was normalized by olomucine, but not after isolomucine or vehicule, administered ei ther in the lateral septum or in the basolateral amygdala. Conclusions: Stress increases the expression of Cdk5 y p35 in brain arcas involved in the processing of emotional information. This enhancement. at least in lateral septum and basolateral amygdala. could be functionally associated with thc gencration of emotional sensitization.