LOW-COST PAPER-BASED (BIO)ELECTROANALYTICAL DEVICES

A. GONZÁLEZ-LÓPEZ; O. AMOR-GUTIÉRREZ; L. BLANCO-COVIÁN; P.I.NANNI; E. COSTA-RAMA; E.NUNEZ-BAJO; MADRID, ROSSANA; M.C. BLANCO-LÓPEZ; M.T. FERNÁNDEZ-ABEDUL

Oviedo

Workshop; II Workshop de ElectroBionet; 2017

Nowadays, miniaturization and reduction of the costs of analytical devices, especially in diagnostic applications, are fields in continuous growth. Portable and easy-to-use devices are produced to reach remote areas where untrained personnel are in charge of the analysis. Electrochemical detection fits properly with these purposes especially when combined with low-cost materials. In this work, we are presenting different devices based on paper and conductive films or common mass-produced materials such as: pins or staples.Firstly, we have designed some bioanalytical devices based on enzymatic reactions. The first one is a maskless biosensor for glucose that uses chromatographic paper and carbon ink as working electrode with gold-plated low-cost connector headers as reference and auxiliary electrodes. [1]. The second approach uses stainless steel pins as electrodes, with this used as working electrode coated with carbon ink. Hydrophobic paper [2] or transparency film can be used as support [3].On the other hand, immunoassays were coupled to a staple-based electrochemical detector for allowing quantitative analysis of human tissue transglutaminase (related to celiac disease) on paper. Secondly, we have developed a novel flow-through assay for pneumolysin. In this case, paper is spotted with capture reagents. After interaction with the target, the intensity of the capture spot can be quantified coupling metallic thin-film electrodes.Finally, paper is not only quite useful for biosensors development, also for designing lab-on-paper devices. In this work, we are also presenting a paper-based device with integrated sample injection, electrophoretic separation and electrochemical detection.