QUANTIFICATION OF BACTERIAL CONTENT IN MILK BY MEANS OF INTERFACE REACTANCE GROWTH CURVES

CARMELO J. FELICE, ROSSANA E. MADRID, JUAN M. OLIVERA, VIVIANA I. ROTGER AND MAX E. VALENTINUZZI

JOURNAL OF MICROBIOLOGICAL METHODS

Año: 1999 p. 37 - 42

0167-7012

The impedancimetric method is a technique for the rapid evaluation of milk bacterial content and also of its subproducts. Several authors have made use of culture conductance changes during bacterial growth for quantitative and qualitative assessments of microbial growth. However, interface capacitance curves, Ci, have not been used. In this paper, we quantify bacteria in cow raw milk by following their growth as the above-mentioned capacitance change time course event. With it, bigger growth variations, shorter detection times and a better coefficient of correlation with the plate count method were obtained than those yielded by conductance curves. Calibration was performed by plotting initial known concentrations, IC [CFU/ml], as a function of the time detection theshold (TDT).