INVESTIGADORES
MADRID Rossana Elena
artículos
Título:
ASPARAGINE QUANTIFICATION IN CELLULAR CULTURE MEDIA USING COPPER MODIFIED CARBON NANOTUBES COMPOSITE ELECTRODES
Autor/es:
MARIA LORENA LĂ“PEZ RODRIGUEZ; GUILLERMINA L. LUQUE; CARMELO J. FELICE; NANCY F. FERREYRA; ROSSANA E. MADRID; GUSTAVO A. RIVAS; CARLA E. GIACOMELLI
Revista:
SENSORS AND ACTUATORS B-CHEMICAL
Editorial:
ELSEVIER SCIENCE SA
Referencias:
Año: 2011 vol. 158 p. 423 - 426
ISSN:
0925-4005
Resumen:
Direct quantification of Asparagine (Asp) in a Streptomyces sp. M7 (SM7) culture medium was performed using multi-wall carbon nanotubes composite electrodes modified with copper microparticles (CNTPECu). A sensitive and fast response is achieved at CNTPE-Cu after additions of SM7 cultures supernatant, without interference of the culture medium components. This response allows determining the Asp concentration as a function of the bacteria growth. The results reveal that the SM7 bacteria consume Asp as a carbon and nitrogen sources in the absence of another carbon source such as glucose.