Protective effect of monoterpene Isoespintanol in a rat model of prediabetes induced by fructose.

DI SARLI GUTIÉRREZ L; CASTRO MC; FARROMEQUE VÁSQUEZ S; VILLAGARCÍA H; GONZÁLEZ ARBELÁEZ LF; ROJANO B; SCHINELLA G; MAIZTEGUI B; FRANCINI F

Pharmaceuticals

Multidisciplinary Digital Publishing Institute (MDPI)

Lugar: Basel; Año: 2024 vol. 17

High-fructose diet (HFD) induces murine alterations like those recorded in human prediabetes. Protective effects of isoespintanol (monoterpene isolated from Oxandra cf xylopioides) on changes induced by HFD were evaluated. Animals were maintained for 21 days with standard diet (C), 10% fructose (F) and F plus isoespintanol (FI, 10 mg/kg, i.p.). Glycemia, triglyceridemia, total and HDL-cholesterol, and insulin resistance index (IRX) were determined. Intraperitoneal glucose tolerance test (IGTT) was performed. In liver, we measured glycogen, lipogenic gene expression (SREBP-1c, GPAT, FAS and CPT1), oxidative stress (GSH and 3´-nitrotyrosine content), inflam-mation markers (iNOS, TNF-α, and PAI-1 gene expression; iNOS, COX-2 protein levels), p-eNOS, p-Akt and p-GSK3β protein levels. Isoespintanol corrected enhanced triglycerides, lipogenic genes, IRX and reduced HDL-cholesterol induced by HFD. Increased liver glycogen and inflam-matory markers and decreased GSH, p-Akt and p-GSK3β measured in F rats were reversed by isoespintanol, p-eNOS/e-NOS and iNOS/GADPH ratios were normalized (p