INTERACTIONS BETWEEN HYBRID NANOPARTICLES DESIGNED BY RADIO-SYNTHESIS AND THE HEMATOLOGY SYSTEM

FLORES, CONSTANZA; ACHILLI, ESTEFANIA; GRASSELLI, M.; CASTELLO, ALEJANDRO

Mar del Plata

Congreso; REUNIÓN ANUAL DE SOCIEDADES DE BIOCIENCIA 2019; 2019

SAIC

Abstract/Resumen: Nanoparticles (NPs) are extremelypromising due to their physicochemical properties and theirdistinctive features for therapeutic applications. When NPs enterthe bloodstream, they immediately interact with plasma andhematology cells (erythrocytes, monocytes and platelets). In thecase of blood plasma, the surface of the NPs interacts withseveral different biomolecules, mostly proteins, formingirreversible layers called the `protein corona´. This adsorption ofproteins onto NPs modify the diverse physicochemical propertiesof NPs such as size, surface charge, surface composition, andfunctionality, hence giving NPs a new biological identity anddifferent biological responses. The aim of this work is todetermine the effect of hybrid NPs on the formation of theprotein corona in vitro and erythrocyte interaction andcoagulation time. These hybrid NPs are AuNPs coated withhuman serum albumin (Alb) multilayers by a novel radiationinduced crosslinking process and are called Au/Alb core/shellnanoparticles (Au/Alb NPs). Albumins from human serum wereadded to the AuNPs suspension, 30% v/v ethanol was added andthen these NPs were irradiated at 10 kGy with a gamma sourceto induce protein crosslinking. The protein corona effect wasevaluated in terms of the hydrodynamic diameter (HD) of theNPs, isolation of the corona complex by size exclusionchromatography and polyacrylamide gel electrophoresis. Inaddition, not changes in the HD were found. In conclusion, ourresults suggest that Au/Alb NPs show a better hemocompatibilitythan albumin monolayer and pegilated Au.