Crystallization and Preliminary Diffraction Studies of a Human Fabm with Anti-I Activity.

CAUERHFF, A; POLIKARPOV I.; MATHOV I; ABATANGELO C.; PLOTKIN L.; GOLDBAUM F. A.; LEONI J.

PROTEIN AND PEPTIDE LETTERS

BENTHAM SCIENCE PUBL LTD

Lugar: Oak Park; Año: 1998 vol. 5 p. 177 - 180

0929-8665

A Fabm from a human monoclonal cold agglutinin (KAU) was obtained by tryptic digestion and purified to homogeneity by FPLC. Crystals were grown by hanging drop vapour diffusion technique against mother liquor containing Sodium Hepes pH 7.5, and PEG 8000 as precipitant. Crystals were found to belong to the trigonal space group P3121(P3221) with the cell parameters a=b=114.233 Å, c=172.787 Å. The crystals diffracted at least to 2.8 Å resolution at a synchrotron beamline.