In vitro assessment of a resorbable scaffold for skin tissue engineering

ANA GONZALEZ WUSENER; IGNACIO RUIZ; CARLOS ARREGUI; ALBERTO BOLGIANI; ELIDA HERMIDA

Paestum (Salerno)

Conferencia; EMBO Conference: The molecular and cellular basis of Regeneration and Tissue Repair; 2016

EMBO

In vitro assessment of a resorbable scaffold for skin tissue engineeringGonzález Wusener AE*, Ruiz IE+~, Arregui CO*, Bolgiani AN°, Hermida EB+~* Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECH);Universidad Nacional de San Martín; Consejo Nacional de Investigaciones Científicas y Técnicas;Buenos Aires, Argentina; + Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín,San Martín, Argentina; ~ Consejo Nacional de Investigaciones Científicas y Técnicas; Buenos Aires,Argentina; ° Centro de Excelencia para Asistencia de Quemaduras (CEPAQ) Fundación Benaim,Hospital Alemán, Buenos Aires, Argentina.PurposeOne of the major applications of tissue-engineered skin scaffolds is to promote the healing of acute and chronic wounds. Once set onto the wound bed and seeded with keratinocytes, skin scaffolds act as a template for dermal and epidermal formation. Among the aspects mediating healing, the main mechanisms of action are: (i) colonization of the scaffold by fibroblasts, (ii) provision of a suitable substrate for the attachment and proliferation of keratinocytes, and (iii) transfer of growth factors from the fibroblasts of the wound bed to enhance keratinocytes proliferation and differentiation, without direct interaction of the two cell types. The aim of thiswork is to assess: the cytotoxicity of the scaffolds, the migration of fibroblasts through thescaffold, and the proliferation of keratinocytes seeded onto the scaffolds.MethodsIn vitro assessments were performed in PHBV scaffolds developed and optimized at our lab. Cytotoxicity tests were performed according to the ISO 10993-5 standard. Adhesion and proliferation assays were performed with murine and human cells, analyzed with fluorescence microscopy and quantitative methods.ResultsResorbable membranes did not induce cytotoxic effects on cells in culture. Moreover, the scaffolds promoted adhesion and proliferation of keratinocytes in vitro, as well as fibroblasts.ConclusionThe 3D porous scaffolds produced in our lab achieve the main features of scaffolds suitable for skin regeneration.