In Vitro Assessment Of Resorbable Scaffold For Skin Tissue Engineering

ANA GONZALEZ WUSENER; IGNACIO RUIZ; CARLOS ARREGUI; ALBERTO BOLGIANI; ELIDA HERMIDA

Washington

Congreso; American Chapter of TERMIS 2014; 2014

Tissue Engineering and Regenerative Medicine International Society

One of the major applications of tissue-engineered skin scaffolds is to promote the healing of acute and chronic wounds. Once set onto the wound bed and seeded with keratinocytes, skin scaffolds act as a template for dermal and epidermal formation. Among the aspects mediating healing, the main mechanisms of action are: (i) colonization of the scaffold by live fibroblasts, (ii) provision of a suitable substrate for the attachment and proliferation of keratinocytes, and (iii) transfer of growth factors from the fibroblasts of the wound bed to enhance keratinocytes proliferation and differentiation, without direct interaction of the two cell types. The aim of this work is to assess: the cytotoxicity of the scaffolds, the migration of fibroblasts through the scaffold, and the proliferation of keratinocytes seeded onto the scaffolds. The conclusion is the feasibility of 3D porous scaffolds produced in our lab to achieve the main features of scaffolds suitable for skin regeneration. Acknowledgments: This work has been partially supported by FONARSEC, Project EMPRETECNO No 37, the National University of San Martı´n (UNSAM) and the National Council of Scientific and Technical Research (CONICET).