INVESTIGADORES
HERMIDA Elida Beatriz
artículos
Título:
Biodegradation and histological response of PHBV porous scaffolds
Autor/es:
ELIDA HERMIDA; IGNACIO RUIZ; ALICIA BALDESSARI; ERICA KREIMANN; RÓMULO CABRINI; GUILLERMO JUVENAL; GLADYS HERMIDA; MANUEL FUNGUEIRO
Revista:
BIOCELL
Editorial:
INST HISTOL EMBRIOL-CONICET
Referencias:
Lugar: Mendoza; Año: 2012 vol. 36 p. 9 - 9
ISSN:
0327-9545
Resumen:
Temporary scaffolds for tissue engineering require bioabsorbable
materials that degrade into non-toxic substances once the tissue is
repaired. Furthermore, these materiales must have suitable mechanical
properties to prevent friability, adapt to anatomical contours, facilitate
handling by surgeons and last but not least, a proper surface
to enhance the adhesion, motility and proliferation of cells and the
interaction with the surrounding tissue. Since polyhydroxyalkanoates
satisfy these properties, many researchers have focused on them to
build up bioabsorbable scaffolds to be harvested with osteoblasts,
fibroblasts, vascular cells, among others. One of the drawbacks of
these biopolyesters, particularly polyhydroxybutyrate-cohydroxyvalerate
(PHBV), is the slow bioabsorption rate, which affects
negatively the formation of the new tissue. Thus, the aim of
this work is to evaluate how the degradation rate depends on the
meso-structures and the media; measurements include dry weight
of samples stored at 37ºC and pH 7.4 in phosphate buffered saline
(PBS) and PBS with lipases as well as changes in the molecular
weight of PHBV. Furthermore, from H&E-stained sections, the toxicity
and inflammatory response after subcutaneous implantation
of porous PHBV scaffolds on rats at 1 week, 1 month and 2 and
half month post implantation are determined.