INVESTIGADORES
GIAMBARTOLOMEI Guillermo Hernan
artículos
Título:
Brucella abortus Omp19 recombinant protein subcutaneously co-delivered with an antigen enhances antigen-specific T helper 1 memory responses and induces protection against parasite challenge.
Autor/es:
CORIA L. M., IBAÑEZ A. E., PASQUEVICH K. A., COBIELLO P. L., FRANK F. M., GIAMBARTOLOMEI G. H., CASSATARO J.
Revista:
VACCINE
Editorial:
ELSEVIER SCI LTD
Referencias:
Lugar: Amsterdam; Año: 2016 vol. 34 p. 430 - 437
ISSN:
0264-410X
Resumen:
The discoveryofeffectiveadjuvantsformanyvaccinesespeciallythosewithlimitedcommercialappeal,such asvaccinestopoverty-relateddiseases,isrequired.Inthiswork,wedemonstratedthatsubcutaneousco-administration ofmicewiththeoutermembraneproteinU-Omp19from Brucella spp. plusOVAasantigen (Ag)increasesAg-specificTcellproliferationandThelper(Th)1immuneresponses in vitro andin vivo. U-Omp19treateddendriticcellspromoteIFN- production byspecificCD4+ T cellsandincreasesT cellproliferation.U-Omp19co-administrationinducestheproductionofAgspecificeffectormemoryTcell populations(CD4+ CD44high CD62Llow T cells).Finally,subcutaneousco-administrationofU-Omp19with Trypanosoma cruzi Ags confersprotectionagainstvirulentparasitechallenge,reducingparasitemiaand weightlosswhileincreasingmicesurvival.TheseresultsindicatethatthebacterialproteinU-Omp19when deliveredsubcutaneouslycouldbeasuitablecomponentofvaccineformulationsagainstinfectiousdiseases requiringTh1immuneresponses.
