Improvement of transfection in DNA Vaccines Against Bovine Herpes Virus 1 by Mannosylation

J.S. PAPPALARDO; A.L. ZAMIT; S. DI GIACOMO; M. TONIUTTI; E. LIMA JR.; M. VASQUEZ MANSILLA; T. MUSACCHIO; C. LANGELLOTTI; V. QUATTROCCHI; V. OLIVERA; R.D. ZYSLER; P.I. ZAMORANO; T.S. LEVCHENKO; V.P. TORCHILIN

Maryland

Conferencia; 38th Annual Meeting & Exposition of the Controlled Release Society; 2011

The Controlled Release Society

Dendritic cells (DC) are the key linkers between innate and adaptative immune response. Manipulation of these cells by DNA targeted transfection is an interesting alternative to obtain better immune responses upon vaccination.Here we show an approach of targeting DC by using a mannosamine derivative anchored to liposomes, micelles and superparamagnetic iron oxide nanoparticles (SPION). Even though SPION showed to have higher binding to DC than liposomes, further toxicological studies need to be done.Mannosylated liposomes (Man-L) were selected to vehicularize plasmids encoding for the glycoprotein D (gD) of BoHV-1. DC targeting improved the immune response in bovines against the virus. From animals challenged with the virus, the group vaccinated with DC-targeted vaccines released minor viral titers 7 days post challenge in comparison with control groups.