HIGH FAT DIETS MODIFY PLASMA LIPID LEVELS AND INTERACTION BETWEEN NORADRENALINE- AND ANGIOTENSIN II-RESPONSES IN RABBITS

SIERRA L; SCACHI FABRICIO; MEDINA MIRTA; SAAD DE SCHOOS S; MARÍA PERAL DE BRUNO; JEREZ S.

Rosario

Congreso; LXI Reunión Anual de la Sociedad Argentina de Farmacología Experimental (SAFE); 2009

Sociedad Argentina de Farmacología Experimental (SAFE)

HIGH FAT DIETS MODIFY PLASMA LIPID LEVELS AND INTERACTION BETWEEN NORADRENALINE- AND ANGIOTENSIN II-RESPONSES IN RABBITS. Sierra L, Scacchi F, Medina M, Saad S, Peral M and Jerez S. INSIBIO (UNT-CONICET). sierraliliana@arnet.com.ar Alterations in plasma lipid levels exert an important impact on vascular function. The aim of this work was to study the effect of different high fat diets on the plasma lipid levels and vascular interactions between angiotensin II (Ang II) and noradrenaline (NA) in rabbit aortic rings. Rabbits were feed with either normal rabbit chow (CD) or a diet cointaining 1% cholesterol for 6 weeks (HD) or a fat diet for 12 weeks (FD). CT, LDL, HDL, TG and free fat acids were measured. Thoracic aorta was excised. Rings were cut and mounted in an organ bath to register isometric contractions. One cumulative dose response curves (CDRC) to Ang II or NA were performed. Arteries were washing and a CDRC to NA or Ang II was performed in the ring previously treated with either Ang II or NA CDRC respectively. Results: CT and LDL were higher in HD. Arachidonic acid was higher in FD and HD. Maximal contractile response (Emax) to Ang II was improved in HD (6124±469mg) and decreased in FD (2877±456mg) with respect to controls (4604±574 mg, p