BIOACTIVATION OF ETHANOL TO ACETALDEHYDE IN DIFFERENT RAT OVARIAN CELLULAR FRACTIONS. ITS MODULATION BY PHYTOCHEMICALS

G.D. CASTRO; A.M.A. DELGADO DE LAYÑO; C. RODRÍGUEZ DE CASTRO; M.H. COSTANTINI; J.A. CASTRO

Montreal, Canadá

Congreso; XI International Congress of Toxicology; 2007

International Union of Toxicology (IUTOX)

Alcohol drinking in women can lead to reproductive deleterious effects. In this study we analyze the possibility that part of them involved ovarian function and were related to ethanol bioactivation to acetaldehyde occurring in situ. We found that ovarian cytosolic fraction metabolize ethanol to acetaldehyde via xanthine oxidoreductase, as suggested by the requirement of a purine cosubstrate and the inhibition by allopurinol. Folic acid, myricetin, hesperetin, ellagic acid, daidzein and quercetin also inhibited this pathway. There is a microsomal process requiring NADPH and oxygen, only inhibible to a minor extent by diethyldithiocarbamate and not inhibible by CO:O2 (80:20) or 1 mM SKF 525A. It was inhibited by 10 uM quercetin and resveratrol, 50 uM esculetin and slightly inhibited by diphenyleneiodonium or curcumin. Enzymes involved are under study. We also detected alcohol dehydrogenase; aldehyde dehydrogenase and xanthine oxidoreductase activities, that showed to be lower compared to those observed in the liver of the same animals. Ultrastructure of ovaries from rats treated with standard Lieber & De Carli liquid diet for 28 days revealed alterations at the level of the granulosa; theca interna and pellucida zones. Results suggest that ovarian metabolism of ethanol to acetaldehyde may play a role in alcohol effects on female reproductive function and that some phytochemicals might be preventive. Supported by SECyT, CONICET and UNSAM.