INVESTIGADORES
CASTRO Gerardo Daniel
artículos
Título:
Interaction of trichloromethyl free radicals with phenylalanine
Autor/es:
G.D. CASTRO; M.I. DÍAZ GÓMEZ; J.A. CASTRO
Revista:
ARCHIVES OF TOXICOLOGY.
Editorial:
Springer-Verlag
Referencias:
Año: 1991 vol. 65 p. 340 - 343
ISSN:
0340-5761
Resumen:
Chemically or enzymatically generated trichloromethyl free radicals interact with liposoluble derivatives of phenylalanine. In vitro, in a chemical system to produce .CCl3 (benzoyl peroxide catalysis), this radical attacked N-acetyl-d,l-phenylalanine methyl ester (PheMeAc) to give a monochlorinated derivative (I) and an unsaturated imine type derivative of PheMeAc (II). Using a liver microsomal system to produce .CCl3 (microsomes + NADPH + CCl4 under nitrogen), the attack of PheMeAc did not result in I or II formation, but in production of benzene. The phenylalanine content in liver microsomal proteins from rats treated with CCl4 6 h before, was not significantly decreased. The results suggest that phenylalanine is a potential target of .CCl3.
