Neurological disorders-associated anti-glycosphingolipid IgG-antibodies display differentially restricted IgG subclass distribution

LARDONE, RICARDO D.; IRAZOQUI, FERNANDO J.; NORES, GUSTAVO A.

Scientific Reports

NATURE PUBLISHING GROUP

Año: 2020 vol. 10

Antibodies against several self-glycans on glycosphingolipids are frequently detected in differentneurological disorders. Their pathogenic role is profusely documented, but the keys for their originremain elusive. Additionally, antibodies recognizing non-self glycans appear in normal human serumduring immune response to bacteria. Using HPTLC-immunostaining we aimed to characterize IgMand IgG subclass antibody responses against glycosphingolipids carrying self glycans (GM1/GM2/GM3/GD1a/GD1b/GD3/GT1b/GQ1b) and non-self glycans (Forssman/GA1/?A? blood group/Nt7) in sera from27 randomly selected neurological disorder patients presenting IgG reactivity towards any of theseantigens. Presence of IgG2 (p = 0.0001) and IgG1 (p = 0.0078) was more frequent for IgG antibodiesagainst non-self glycans, along with less restricted antibody response (two or more simultaneous IgGsubclasses). Contrariwise, IgG subclass distribution against self glycans showed clear dominance forIgG3 presence (p = 0.0017) and more restricted IgG-subclass distributions (i.e. a single IgG subclass,p = 0.0133). Interestingly, anti-self glycan IgG antibodies with simultaneous IgM presence had higherproportion of IgG2 (p = 0.0295). IgG subclass frequencies were skewed towards IgG1 (p = 0.0266)for ?anti-self glycan A? subgroup (GM2/GM1/GD1b) and to IgG3 (p = 0.0007) for ?anti-self glycan B?subgroup (GM3/GD1a/GD3/GT1b/GQ1b). Variations in players and/or antigenic presentation pathwayssupporting isotype (M-G) and IgG-subclass pattern differences in the humoral immune responseagainst glycosphingolipids carrying non-self versus self-glycans are discussed.