A monoclonal antibody against Babesia bovis merozoite surface antigen-2c, suitable for a competitive ELISA test.

DOMINGUEZ, M; ZABAL, O.; WILKOWSKY, S.; ECHAIDE, I.; ASENZO, G.; RODRIGUEZ, A; ZAMORANO, P.; FARBER, M.; SUAREZ, C.; MONICA OFELIA JACOBSEN

Annals of the New York Academy of Sciences

The New York Academy of Sciences

Año: 2004 vol. 1026 p. 165 - 170

Bovine babesiosis caused by Babesia bovis is a disease that hampers the production of beef and dairy cattle in tropical and subtropical regions of the world. New diagnostic methods based on recombinant antigens constitute valuable biotechnological tools for the strategic control of this disease. We have developed a competitive enzyme-linked immunosorbent assay that includes a recombinant form of the merozoite surface antigen-2c and a novel monoclonal antibody against it. Preliminary results showed that this test is able to identify specific antibodies against B. bovis from experimentally and naturally infected cattle. Babesia bovis is a disease that hampers the production of beef and dairy cattle in tropical and subtropical regions of the world. New diagnostic methods based on recombinant antigens constitute valuable biotechnological tools for the strategic control of this disease. We have developed a competitive enzyme-linked immunosorbent assay that includes a recombinant form of the merozoite surface antigen-2c and a novel monoclonal antibody against it. Preliminary results showed that this test is able to identify specific antibodies against B. bovis from experimentally and naturally infected cattle. B. bovis from experimentally and naturally infected cattle.