Exploring magnetic hyperthermia: ferrofluids, in vitro and in vivo experiment

CORAL, D.F.; SOTO, P.A.; MENDOZA SELIS P; PASQUEVICH G; VEIGA, A.; GONZALEZ, S.; SPINELLI, E.; VIVIANA BLANK; FRANCISCO SANCHEZ; CLARA P. SETTON; LEONOR ROGUIN; MARCELA FERNANDEZ VAN RAAP

Gramado

Congreso; XVI SBP MRS Brazil; 2017

SBP MRS Brazil

Magnetic hyperthermia (MH) is a cancer treatment where magnetic nanoparticles(MNP) immersed in radiofrequency (RF) fields, are used to increase carcinoma cells temperature until the apoptotic cellular death mechanism is activated. An important mechanism is the energy transduction from electromagnetic to thermal; this process is governed by the MNP and dispersant matrix properties [1]. Then, MNP properties can be improved by controlling their structuring during the synthesis [2]. In this work, nanoclusters (NC), formed by iron oxide nanoparticleswere synthesized by high temperature co-precipitation. Different nanocluster size, from 20 to 25 nm, were achieved by varying time andinitial synthesis temperature. The response to RF was studied by exposing an aqueous nanocluster suspension to fieldamplitudes between 2 and 54 kA/m and frequencies between 100 and 260 kHz. The NC cytotoxicity was determined by exposing B16 in-vitro cell culture to media doped with NC and MH performance were studied ex-vivo by injecting a NC suspension in mice melanoma tumours. Results show better performance of NC comparing with individual MNP,validating their use in MH therapy. For ex-vivo and in-vitroexperiments, we have used a newly developed device, based on a parallel LC resonant circuit optimized to generatealternating magnetic fields of 100 kHz frequency and amplitude adjustable from 2 to 15 kA/m [3]. We will also present a brief description of this versatile tool for research involving the use of magnetic materials and alternating magnetic field for fighting cancer, like MH and drug/gene delivery triggered by magnetic stimuli.Key words:Nanoclusters, Magnetic hyperthermia, Cancer treatment.References:[1]Fernández van Raap, M. B., Coral, D. F., Yu, S., Muñoz, G. A., Sánchez, F. H., &Roig, A. (2017). Anticipating hyperthermic efficiency of magnetic colloids using a semi-empirical model: a tool to help medical decisions. Physical Chemistry Chemical Physics, 19, 7176-7187.