CONICET | Buscador de Institutos y Recursos Humanos

Peripheral nerve regeneration promoted by Adipose-derived stem cells (AdSC) magneto targetingPaula Soto1; V. Usach1; G. Piñero1; Marcela van Raap2 and P. Setton-Avruj1.1Instituto de Química y Fisicoquímica Biológica ?Alejandro Paladini? UBA-CONICET Facultad de Farmacia y Bioquímica. 2Instituto de Física La Plata, UNLP, CONICET, Facultad de Ciencias Exactas.Wallerian degeneration (WD) is an efficient animal experimental model in mimicking the impact of peripheral nerve lesion and to shed light on possible regeneration strategies. AdSC transplant is a promising tool for regenerative therapies. In culture, AdSC can internalize magnetic nanoparticles (MNP) [1], which can be driven to the lesion site by an external magnetic field.In this context, the aim of the present work was to test whether AdSC-MNP magneto targeting can enhance the regenerative ability of AdSC upon rat sciatic nerve lesion.To this end, cultured AdSC were characterized for multipotent cell markers expression. MNP internalization was evaluated through transmission electron microscopy and vibrating sample magnetometry (VSM) experiments. Likewise, epifluorescence microscopy and VSM analyses were performed to evaluate the arrival of AdSC-MNP at the injured nerve. Finally, through immunofluorescence, western blot and electrophysiological experiments AdSC-MNP transplantation effects on nerve morphology and conduction were evaluated.Our results show that AdSC express CD105, CD90 and CD34, and can internalize between 2-4 pgMNP/cell. We demonstrate AdSC-MNP to supersede AdSC arrival exclusively at the lesion site and exert beneficial effects on nerve morphology and conduction.In short, our results prove AdSC-MNP magneto targeting constitute a valuable tool to supersede AdSC arrival at the lesion site, enhancing nerve regeneration.[1] Fan et al. Stem Cell Research & Therapy 2013, 4:44

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