CONICET | Buscador de Institutos y Recursos Humanos

Remyelination by Bone Marrow Mononuclear Cells: efficiency in cell tracking vs efficiency in cell functionality. Gonzalo Piñero, Vanina Usach and Patricia Setton-Avruj Cátedra de Química Biológica Patológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, IQUIFIB- Instituto Dr Alejandro Paladini, UBA-CONICET, Buenos Aires, Argentina. In order to assess bone marrow mononuclear cell (BMMC) remyelination ability we used a model of sciatic nerve crush. We demonstrated spontaneous migration of endogenous and transplanted wild type BMMCs to the demyelinated nerve. Also, BMMCs were shown to exert a beneficial effect by accelerating the demyelination process and, subsequently, remyelination. In this work, and in order to attain long-lasting cell labeling, we used BMMCs isolated from adult rats of the transgenic strain [Wistar-TgN(CAG-GFP)184ys] (BMMCs-EGFP+) to evaluate their remyelination ability. BMMCs-EGFP+ were transplanted on adult wild type Wistar rats. At different survival times, epifluorescence and confocal microscopy were used to evaluate BMMC-EGFP+ migration and their effect on sciatic nerve remyelination. Results showed BMMC-EGFP+ arrival at the nerve as from 3 days post transplant. In terms of their remyelination effect, results showed a marked decrease in MBP levels (a major myelin protein) as from 5 days after transplant and after 14 days signs of nerve fiber regeneration and remyelination began to be evident. In turn, when comparing BMMC and BMMC-EGFP+ performance, results showed a lower yield in the isolation of BMMCs-EGFP+ and a different migration kinetics for different transplant time points. On the basis of these results, BMMCs-EGFP+ appear to be less efficient than BMMCs in helping the remyelination process , although further studies are required to elucidate the nature of their deficiency.

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