Caracterización histológica del hígado de ratas intoxicadas crónicamente con aluminio. Efecto de la vitamina E

PISANI G.B.; GONZÁLEZ M.; ÁLVAREZ M.L.; ROMA M.G.; LUGANO M.C.; CARRILLO M.C.

Tandil

Congreso; 10º Congreso Argentino de Ciencias Morfológicas; 2006

Sociedad Argentina de Ciencias Morfológicas

Human exposure to Aluminum (Al) has increased. In experimental animals, Al exposure has an impact on biliary secretion mediated by oxidative stress. Our aim was to characterize through a morphometric model, liver histology from Al-chronically intoxicated (Alci) rats, treated or not with Vitamin E (Vit E). Male Wistar rats were divided in groups: Controls (G1); Alci (G2); treated with Vit E (G3) and Alci + Vit E (G4). On liver sections we determinate: the number of bile ducts (NBD) (counted); volume density of portal areas (Vvpa) expressed as percentage (stereologically); hepatocellular necrosis (HN) and portal tract inflammation (PTI) (scoring system); fibrosis percent index (FI) (digital analysis) and apoptotic index (AI) (TUNEL).   G1 G2 G3 G4 NBD 1,20±0,02 2,43±0,41* 1,26±0,07 1,31±0,10 Vvpa 1,30±0,04 4,93±0,72* 1,33±0,06 1,65±0,50 FI 0,60±0,06 1,18±0,21* 0,62±0,11 0,55±0,01 HN   1,0±0.0 2,1±0,4* 1,0±0,0 1,1±0,3 PTI   1,0±0.0 2,4±0,5* 1,1±0,1 1,2±0,4 AI 0,010±0.001 0,046±0,023* 0,010±0.001 0,015±0,005 Mean±SD. One-way ANOVA, following by Tukey-Kramer or DunnÕs test, n=5, *p<0,05 vs G1, G3, G4. Morphometric model used let quantify histological alterations on Alci rats showing that these alterations were reduced when rats were treated with Vit E.