INVESTIGADORES
ELOLA Maria Teresa
artículos
Título:
Glycosylation-dependent binding of galectin-8 to activated leukocyte cell adhesion molecule (ALCAM/CD166) promotes its surface segregation in breast cancer cells
Autor/es:
FERNANDEZ, MARISA M.; FERRAGUT, FÁTIMA; CARDENAS DELGADO, VICTOR M.; BRACALENTE, CANDELARIA; BRAVO, ALICIA INÉS; CAGNONI, ALEJANDRO J.; NUÑEZ, MYRIAM; MOROSI, LUCIANO G.; QUINTA, HÉCTOR R.; ESPELT, MARÍA V.; TRONCOSO, MARIA F.; WOLFENSTEIN-TODEL, CARLOTA; MARIÑO, KARINA V.; MALCHIODI, EMILIO L.; RABINOVICH, GABRIEL A.; ELOLA, MARÍA TERESA
Revista:
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2016
ISSN:
0304-4165
Resumen:
Background: We previously demonstrated that the activated leukocyte cell adhesion molecule (ALCAM/CD166) can interact with galectin-8 (Gal-8) in endothelial cells. ALCAM is a member of the immunoglobulin superfamily that promotes homophilic and heterophilic cell-cell interactions. Gal-8 is a tandem-repeat-type galectin, known as a matricellular protein involved in cell adhesion. Here, we analyzed the physical interaction between both molecules in breast cancer cells. Methods: We performed binding assays by surface plasmon resonance to study the interaction between ALCAM fully-glycosylated ectodomain or endogenous ALCAM from breast cancer cells with Gal-8. We also analyzed the binding of ALCAM-silenced or control breast cancer cells to immobilized Gal-8 by SPR. In internalization assays, we evaluated in the influence of Gal-8 in ALCAM endocytosis. Results: We showed that recombinant and endogenous ALCAM from breast carcinoma cells physically interacted with Gal-8 in a glycan-dependent fashion. Moreover, ALCAM-silenced breast cancer cells exhibited reduced binding to Gal-8 relative to control cells. Importantly, Gal-8 prevented ALCAM internalization by trapping this receptor at the cell surface. Conclusions: Our data indicate that ALCAM and Gal-8 interact at the surface of breast cancer cells. General Significance: A novel ALCAM heterophilic interaction with Gal-8 is demonstrated at the surface of breast cancer cells.
