DIAZ DE ASTARLOA Juan Martin
congresos y reuniones científicas
Astyanax species diversity through DNA barcoding: molecular data assessment and the species complexes
ROSSINI, B.; OLIVEIRA, C.; MELO, F.; BERTACO, V.; DÍAZ DE ASTARLOA, J. M.; ROSSO, J.J.; FORESTI, F.
Mar del Plata
Simposio; IV Simposio Argentino de Ictiología; 2015
Laboratorio de Biotaxonomía Morfológica y Molecular de Peces, IIMyC-CONICET-UNMdP
The molecular identification through DNA barcoding has been extensively used in an attempt to solve taxonomic problems and help in the discovery of new species. The Neotropical fishes exhibits a wide variety of shapes and sizes, with many species yet to be cataloged and many species very difficult to identify. Characidae is the most species rich family of Characiformes, with many genera with taxonomic uncertainties. One of such species rich genus widely distributed and with many taxonomic conflicts is Astyanax. Here we present an extensive analysis of the genus in its almost entirely area of occurrence, based on DNA barcoding. The use of different approaches to clustering sequences (ABGD, GMYC and BIN programs) showed consistency in the results obtained by the initial cutoff value of 2%, but GMYC shows a tendency to identify a larger number of clusters than others. The results point to the existence of four large groups in the genus. However, whereas some groups, as that composed by the trans-Andean species, are composed of well-defined species in others there is a grouping of several species in a single cluster, making the species delimitation very difficult and in many cases impossible. The final results suggest a very complex scenario in the taxonomy of Astyanax.