INVESTIGADORES
ALVAREZ Elida Maria Del Carmen
artículos
Título:
Haomostatic and immune role of cellular clotting in the sipunculan Themiste petricola
Autor/es:
CAVALIERE VICTORIA; PAPADEMETRIO DANIELA; ALVAREZ ELIDA; BLANCO GUILLERMO
Revista:
CELL AND TISSUE RESEARCH
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2010 vol. 339 p. 597 - 611
ISSN:
0302-766X
Resumen:
Sipunculans, a small phylum of coelomated
marine worms closely related to polychaete annelids, lack
a true circulatory system. We have previously shown that
the sipunculan Themiste petricola can form a cellular clot,
without congealing, of cell-free coelomic fluid. The clot is
formed by the aggregation of large granular leukocytes
(LGLs) and may serve not only haemostatic but immune
functions, since dissimilar particles may become entrapped
within it. We have now evaluated the capacity of a massive
clot, induced in vitro by sea water contact, to stop coelomic
fluid flow. We have further studied smaller clots induced on
glass-slides either with or without the presence of bacteria
placed for entrapment within the clot. The fate of clotting
LGLs is cell death while forming a cohesive mass, although
cytoplasmic and nuclear remnants are shed from the clot.
These remnants and any bacteria that avoid clot entrapment
or are detached from the clot are engulfed by non-clotting
cells that include small granular leukocytes (SGLs) and
large hyaline amebocytes (LHAs). Both cell types can be
found other than in the clot but SGLs also occur around
the clot edges heavily loaded with engulfed material.Themiste petricola can form a cellular clot,
without congealing, of cell-free coelomic fluid. The clot is
formed by the aggregation of large granular leukocytes
(LGLs) and may serve not only haemostatic but immune
functions, since dissimilar particles may become entrapped
within it. We have now evaluated the capacity of a massive
clot, induced in vitro by sea water contact, to stop coelomic
fluid flow. We have further studied smaller clots induced on
glass-slides either with or without the presence of bacteria
placed for entrapment within the clot. The fate of clotting
LGLs is cell death while forming a cohesive mass, although
cytoplasmic and nuclear remnants are shed from the clot.
These remnants and any bacteria that avoid clot entrapment
or are detached from the clot are engulfed by non-clotting
cells that include small granular leukocytes (SGLs) and
large hyaline amebocytes (LHAs). Both cell types can be
found other than in the clot but SGLs also occur around
the clot edges heavily loaded with engulfed material.