INVESTIGADORES
ZORREGUIETA Angeles
congresos y reuniones científicas
Título:
A novel B. suis Adhesin identified by Phage Display mediates Bacterial Adhesion to Epithelial cells
Autor/es:
POSADAS, D. M.; RUIZ, V.; MARTÍN, F A; ZORREGUIETA, A
Lugar:
Puerto Madryin
Reunión:
Congreso; XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2010
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Brucella is an intracellular pathogen responsible
for the zoonotic disease called brucellosis. Adhesins are important virulence
factors in bacterial infections. However,little is known about these molecules in brucellaceae. In an attempt to
identify genes coding for adhesins to some key components of the host
extracellular matrix molecules, a phage display library of fragmented genomic
DNA from B. suis was prepared in
pG8SAET vector. Three genes encoding putative Fibronectin-binding molecules
were identified by using panning procedures. Binding specificity to fibronectin was confirmed by ELISA and
panning experiments for all candidates. One of these candidates, designated
BmaC, belongs to the type I autotransporter protein family. Phages displaying fusions
to BmaC peptides were able to adhere to culture cells and blocked the binding
of B. suis to HeLa cells in a
dose-dependent manner. A deletion mutant in the bmaC gene was obtained, and adhesion capability in this strain was
determined. In co-infection experiments of
epithelial cells the wild type
strain out-competed a bmaC-deletion mutant showing that bmaC contributes to adherence to model
cells. Biochemical and microscopy studies suggest that BmaC is a
surface-exposed adhesin, localized in the outer membrane of the bacteria. BmaC may have a crucial role in the
interaction of B. suis with its host.