INVESTIGADORES
ZORREGUIETA Angeles
congresos y reuniones científicas
Título:
Interaction between biofilm matrix components in Rhizobium leguminosarum
Autor/es:
ABDIAN, P; CARAMELO, J.; VOZZA, N; VON BILDERLING C.; RUSSO, D. M.; PIETRASANTA, LI; ZORREGUIETA, A
Lugar:
Puerto Varas
Reunión:
Congreso; XII Congreso PABMB, XLIX Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB), XXXVI Reunión de la Sociedad de Bioquímica y Biología Molecular de Chile, LVI Reunión anual de la Sociedad de Biología de Chile, IV Re; 2013
Institución organizadora:
Sociedad de Bioquímica y Biología Molecular de Chile
Resumen:
Rhizobium spp. are soil bacteria
that infect root tissue and induce the development
of nodules on leguminous plants under conditions of nitrogen starvation. The ability of rhizobia to grow in varied
environmental conditions is probably due to its ability to produce different
types of biofilm. The main component of the extracellular matrix of the biofilm
developed on a glass surface, is an acidic exopolysaccharide or EPS that provides
support to the biofilm structure. Proteins secreted by a type I secretion
system (PrsDE) have been shown to be important for biofilm development.
Interestingly, some of them possess one o more characteristic Ra/CHDL (cadherin
like-) domains that confer carbohydrate binding ability. Among them, we have
characterized RapA2, a unipolar calcium binding lectin that specifically
recognizes the EPS and the glycanases Ply that regulate the length of EPS
molecules in the extracellular medium. A characteristic distribution of EPS
molecules length is important for the development of an ordered polysaccharide
network. Thus, Ply mutants are unable to form a typical biofilm. On the other
hand, anomalous levels of RapA2 affect the production of surface acidic
polysaccharides. A model depicting the interaction of RapA2, Ply glycanases and
acidic polysaccharides during the progress of biofilm matrix development is
presented. Supported by CONICET and Agencia de Promoción Científica y
Tecnológica-FONCYT