INVESTIGADORES
ZORREGUIETA Angeles
artículos
Título:
Inhibition of Cell Division Induced by External Guide Sequences (EGS Technology) Targeting ftsZ.
Autor/es:
DAVIES SALA, C.; SOLER BISTUE, A J C; KORPRAPUN, L; ZORREGUIETA, A; TOLMASKY, M E
Revista:
PLOS ONE
Editorial:
PUBLIC LIBRARY SCIENCE
Referencias:
Lugar: San Francisco; Año: 2012 vol. 7 p. 1 - 5
ISSN:
1932-6203
Resumen:
EGS (external guide sequence) technology is a promising approach to
designing new antibiotics. EGSs are short antisense oligoribonucleotides
that induce RNase P-mediated cleavage of a target RNA by forming a
precursor tRNA-like complex. The ftsZ mRNA secondary structure was
modeled and EGSs complementary to two regions with high probability of
being suitable targets were designed. In vitro reactions showed that
EGSs targeting these regions bound ftsZ mRNA and elicited RNase
P-mediated cleavage of ftsZ mRNA. A recombinant plasmid, pEGSb1, coding
for an EGS that targets region "b" under the control of the T7 promoter
was generated. Upon introduction of this plasmid into Escherichia coli
BL21(DE3)(pLysS) the transformant strain formed filaments when
expression of the EGS was induced. Concomitantly, E. coli harboring
pEGSb1 showed a modest but significant inhibition of growth when
synthesis of the EGSb1 was induced. Our results indicate that EGS
technology could be a viable strategy to generate new antimicrobials
targeting ftsZ.