INVESTIGADORES
ZORREGUIETA Angeles
artículos
Título:
BtaE, an Adhesin That Belongs to the Trimeric Autotransporter Family, Is Required for Full Virulence and Defines a Specific Adhesive Pole of Brucella suis
Autor/es:
RUIZ-RANWEZ, M.V.; POSADAS, D. M.; VAN DER HENST, C.; ESTEIN, S.; ABDIAN, P.; AROCENA, G.M.; MARTÍN, F A; SIEIRA, R.; DE BOLLE, X.; ZORREGUIETA, A
Revista:
INFECTION AND IMMUNITY
Editorial:
AMER SOC MICROBIOLOGY
Referencias:
Lugar: Washington; Año: 2013 vol. 81 p. 996 - 1007
ISSN:
0019-9567
Resumen:
Brucella is responsible for brucellosis, one of the most common zoonoses
worldwide that causes important economic losses in several countries.
Increasing evidence indicates that adhesion of Brucella spp. to host
cells is an important step to establish infection. We have previously
shown that the BmaC unipolar monomeric autotransporter mediates the
binding of Brucella suis to host cells through cell-associated
fibronectin. Our genome analysis shows that the B. suis genome encodes
several additional potential adhesins. In this work, we characterized a
predicted trimeric autotransporter that we named BtaE. By expressing
btaE in a nonadherent Escherichia coli strain and by phenotypic
characterization of a B. suis ΔbtaE mutant, we showed that BtaE is
involved in the binding of B. suis to hyaluronic acid. The B. suis ΔbtaE
mutant exhibited a reduction in the adhesion to HeLa and A549
epithelial cells compared with the wild-type strain, and it was
outcompeted by the wild-type strain in the binding to HeLa cells. The
knockout btaE mutant showed an attenuated phenotype in the mouse model,
indicating that BtaE is required for full virulence. BtaE was
immunodetected on the bacterial surface at one cell pole. Using old and
new pole markers, we observed that both the BmaC and BtaE adhesins are
consistently associated with the new cell pole, suggesting that, in
Brucella, the new pole is functionally differentiated for adhesion. This
is consistent with the inherent polarization of this bacterium, and its
role in the invasion process.