INVESTIGADORES
GONZALEZ FLECHA Francisco Luis
artículos
Título:
Oligomerization of the plasma membrane calcium pump involves two regions with different thermal stability
Autor/es:
VALERIA LEVI; JUAN PABLO F.C. ROSSI; PABLO R. CASTELLO; F. LUIS GONZÁLEZ-FLECHA
Revista:
FEBS LETTERS
Editorial:
Elsevier
Referencias:
Año: 2000 vol. 483 p. 99 - 103
ISSN:
0014-5793
Resumen:
Ca(2+) pump dimerization was studied by using a combined approach of
thermal denaturation and fluorescence resonance energy transfer. The
measurement of calcium pump ability to dimerize after the unfolding of
individual functional domains of the enzyme demonstrated the existence
of two different regions involved in the self-association process. One
of these regions is highly susceptible to thermal unfolding and was
identified as the calmodulin (CaM)-binding domain. The other region
whose thermal stability is higher than those of the catalytic and
CaM-binding domains could be related with the previously found
C28W-binding regions.
