Pseudomonas extremaustralis sp. nov. a polyhydroxybutyrate-producer isolated from Antarctic environments

LÓPEZ, N.I; PETTINARI MJ,; STACKEBRANDT, E.; TRIBELLI, P.M.; POTTER, M; STEINBUCHEL, A.; MÉNDEZ, B.S.

CURRENT MICROBIOLOGY

Springer Verlag, New York

Año: 2009 vol. 59 p. 514 - 519

0343-8651

Abstract A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3T) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3T was shown to belong to the genus Pseudomonas sensu stricto. Physiological and biochemical tests supported the phylogenetic affiliation. Strain 14-3T is closely related to Pseudomonas veronii DSM 11331T, sharing 99.7 % sequence similarity. DNA-DNA hybridization experiments between the two strains showed only moderate reassociation similarity (35.1%). Tests for arginine dihydrolase and nitrate reduction were positive, while those for denitrification, indol production, glucose acidification, urease, ßgalactosidase, esculin, caseine and gelatin hydrolysis were negative. Growth of thisbacterium occurred in a range from 4 to 37° C but not at 42°C. It accumulated poly(3-hydroxybutyrate) when grown on sodium octanoate medium. Strain 14-3T thereforerepresents the type strain of a new species, for which the name Pseudomonasextremaustralis sp. nov. is proposed. The type strain 14-3T has been deposited as DSM17835T and as CIP 109839T.