rpoS gene expression in carbon-starved cultures of the polyhydroxyalkanoate accumulating species Pseudomonas oleovorans

RUIZ, JA; LÓPEZ, N.I; MÉNDEZ, B.S.

CURRENT MICROBIOLOGY

Springer-Verlag New York

Año: 2004 vol. 48 p. 396 - 400

0343-8651

Abstract. The expression of the rpoS gene during PHA depolymerization was monitored in Pseudomonasoleovorans GPo1 and its mutant defective in PHA degradation by analyzing the tolerance tooxidative and thermal stresses and the RpoS intracellular content. An increase in the tolerance to H2O2 and heat shock was observed coincidentally with PHA degradation. Western blotting experiments performed in carbon-starved cultures showed that the RpoS levels were higher in the wild type than in the mutant strain. Complementation of the phaZ mutation restores the wild-type RpoS levels. These results suggest a probable association between PHA depolymerization and the stress tolerance phenotype controlled by RpoS.