165) Efecto de la administración de ketamina en dosis subanestésicas sobre la actividad metabólica del hipocampo a los 8 minutos y a las 24 horas (estudio preliminar).

GUEVARA MA; GARCIA MENENDEZ, S.; MAZZEI, L.; ROMANOWICZ, E.; BARRUTIETA, I.; VAQUERO, A.; BAIARDI, G.; LAFUENTE, J.V.; GARGIULO, P.A.

Mendoza

Congreso; XXXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo.; 2021

Sociedad de Biología de Cuyo.

165) Guevara, M.A.; García Menéndez, S.; Mazzei, L.; Romanowicz, E.; Barrutieta, I.; Vaquero, A.; Baiardi, G.; Lafuente, J.V.; Gargiulo, P.A. Efecto de la administración de ketamina en dosis subanestésicas sobre la actividad metabólica del hipocampo a los 8 minutos y a las 24 horas (estudio preliminar). XXXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo. Realizado en forma virtual los días 6 y 7 de Noviembre de 2021.- EFFECT OF THE ADMINISTRATION OF KETAMINE IN SUBANESTHETIC DOSES ON THE METABOLIC ACTIVITY OF THE HIPPOCAMPUS AT 8 MINUTES AND 24 HOURS (PRELIMINARY STUDY)Guevara MA1, García Menendez S1, Mazzei L4, Romanowicz E1, Barrutieta Arberas I2, Vaquero A2, Baiardi G, Lafuente, JV2, Gargiulo PA11 Laboratory of Neurosciences and Experimental Psychology. Area of Pharmacology. Faculty of Medical Sciences. National University of Cuyo. (CONICET).   2. Laboratory of Clinical and Experimental Neurosciences (LaNCE). Department of Neuroscience. University of the Basque Country. Leioa. Spain.  3 Laboratory of Neuropharmacology. Faculty of Chemical Sciences, Catholic University of Cordoba. 4. Laboratory of Basic and Translational Experimental Pharmacology. Area of Pharmacology. Faculty of Medical Sciences. National University of Cuyo. (CONICET).   E- mail: vetguevara@hotmail.comThe information available on the changes in brain metabolic activity under the effect of ketamine is very varied and contradictory. Some authors report an increase in brain metabolism and other studies report a decrease in said activity. In previous works we determined that the administration of ketamine in subanesthetic doses produces a decrease in mitochondrial metabolic activity as measured by the MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) assay in spectrophotometer at 570 nm in the hippocampus of male rats. This reduction occurs in the metabolism of mitochondrial oxide reduction. In the present study, we decided to investigate whether the decrease in hippocampal metabolism in rats treated with ketamine occurs temporarily or permanently. The following experiment has CICUAL protocol approval (97/2017 and 128/2018). Male Holtzman strain rats weighing 240-290 g (n = 5 per dose) were used. The animals were divided into 2 groups. The first group received the drug and 8 minutes later they were sacrificed by decapitation. The hippocampus was extracted and the MTT test was carried out. The second group was sacrificed at 24 hours and the MTT test was applied to hippocampi. In each group, the doses used were administered intraperitoneally (1.25, 2.5, 5, 10, 15 and 20 mg/kg) and compared to saline control. Significance was studied using the ANOVA I test followed by the Student-Newman-Keuls post-test. In all cases a value of p