158) ACUTE CEREBRAL BEHAVIORAL AND METABOLIC KETAMINE (N-METHYL-D-ASPARTATE RECEPTOR ANTAGONIST) EFFECTS IN RATS INTRAPERITONEALLY IN PASSIVE AVOIDANCE TEST. PARTIAL RESULTS.

LORENZO, S.; MARTIN CUNIETTI, J.; GARGIULO, P.A.; GUEVARA, M.A.

Mendoza

Simposio; IIº International Symposium of Translational Medicine. XII Annual Meeting of Investigation at the Faculty of Medical Sciences. Mendoza. 7 a 8 de marzo de 2019.-; 2019

Facultad de Ciencias Médicas. Universidad Nacional de Cuyo.

158) Lorenzo Acquaro S, Martín Cunietti J, Gargiulo P, Guevara M. Acute cerebral behavioral and metabolic ketamine (N-methyl-D-aspartate receptor antagonist) effects in rats intraperitoneally in passive avoidance test. Partial results. IIº International Symposium of Translational Medicine. XII Annual Meeting of Investigation at the Faculty of Medical Sciences. Mendoza. 7 y 8 de marzo de 2019.- Introduction: Phencyclidine (antagonist of the N-methyl-D-aspartic acid (NMDA) receptors), induces a schizophrenic-like psychosis in humans. Ketamine is a general anesthetic, NMDA antagonist, which at subanesthetic doses induces psychotiform manifestations. We study experimentally it in rats in our laboratory. Hypothesis: Ketamine subanesthetic doses may induce brain metabolic and behavioarl disturbances in rats that could be considered animal models of psychosis. Objectives: The objective of present study is to verify metabolic alterations in prefrontal cortex and hippocampus, by ketamine effect and to correlate those doses with alterations in cognition (passive avoidance). Methods: Passive avoidance test Holtzman male rats (240-280 g) in two groups (n=20): Saline and Ketamine controls (2.5 mg / kg), injected 8 min before the test to interfere with the acquisition. The metabolic activity was measured by the MTT test (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) through the spectrophotometric measurement of the wavelength. Two groups were used: Saline Control (n = 5) and Ketamine (2.5 mg / kg, n = 5). The brain structures to be studied were removed (hippocampus and prefrontal cortex) 8 minutes after the drug or saline was applied. Results: We observed a shorter latency time 1 (p