144) Ketamine disrupts working memory in a hole board test and the metabolic activity in prefrontal cortex and nucleus Accumbens.

GUEVARA, M. ; MARQUEZ HERRERO, S. ; GARCIA MENENDEZ, S. ; GARGIULO, P.A.

Mendoza.-

Congreso; XXXIV Reunión Científica Anual de la Sociedad de Biología de Cuyo.; 2016

Sociedad de Biología de Cuyo.-

144) KETAMINE DISRUPTS WORKING MEMORY IN A HOLE BOARD TEST AND THEMETABOLIC ACTIVITY IN PREFRONTAL CORTEX AND NUCLEUS ACCUMBENSGuevara M, Marquez Herrero S, Garcia Menendez S, Gargiulo PA. Laboratory of Neurosciences and Experimental Psychology. Area of Pharmacology. Faculty of Medical Sciences. National University of Cuyo. (CONICET). E-mail: vetguevara@hotmail.comKetamine, a N-methyl-D-Aspartic-acid (NMDA) receptor antagonist, is an anesthetic that has psychotogenic effects, and it led to use it inducing animal models of schizophrenia. In the present study the effects of systemic administration ketamine (1; 2,5; 2,5; 5; 10; 15 y 20 mg/Kg ip) were evaluated in a hole board test (HBT). Besides the metabolic activity of the Pre-Frontal Cortex (PFC) and the Nucleus Accumbens Septi (NAS) were simultaneously evaluated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) through wavelength measurement using a spectrophotometer. Male rats derived from a Holtzman colony weighing 240-290 g were used. Hole exploration strategies and locomotor activity were significantly modified in the HBT. Non ambulatory movements were significantly decreased at the dose of 10 mg/kg (p