38) Effects of Single Injection of Naloxone and DAMGO within Nucleus Acumbens Septi in the Plus Maze Test in rats.

MORSUCCI, CRISTIAN; OKASOVA, AINURA; MULET, DANIELA; GALIANA, GRACIANA; RODRÍGUEZ, VANESA; BAIARDI, GUSTAVO; LAFUENTE, JOSÉ VICENTE; ELIAS, PABLO ADOLFO; LANDA, ADRIANA INES; SOAJE, MARTA; GARGIULO, P.A.

ACTA POLONIAE PHARMACEUTICA

POLSKIEGO TOWARZYSTWA FARMACEUTYCZNEGO

Año: 2018

0001-6837

ACCEPTED DECEMBER 2016. Effects of single injection of Naloxone and DAMGO within Nucleus Acumbens Septi in the plus maze test in rats. Nucleus Accumbens Septi (NAS) is studied because its relations with cognition and anxiety. Its pharmacological manipulation is widely used in experimental psychopathology to reproduce psychotic signs and symptoms in animal models. In the present study the effect of the injection of an agonist and a μ-receptor antagonist in this structure is assessed. Holtzman strain male rats (240-290 g) were cannulated bilaterally in NAS. One week after the injection they were subjected to an anxiety test, prior saline injection (controls), DAMGO ([D-Ala2, N-MePhe4, Gly-ol] -encephalin, opioid agonist) or naloxone (opioid antagonist). We evaluated the set of parameters classically considered in our laboratory (open arm time, time per entry, open arm entries, closed arm entries, open/closed arm quotient, open and closed arm ends arrivals, rearing, fecal bowls and grooming behaviors. There was only a significant increase in the length of stay in the open arm with the injection of DAMGO (0.2 μg / 1 μl, p