INVESTIGADORES
BRAVO ALMONACID Fernando Felix
artículos
Título:
Rapid inmunological detection of potato viruses in plant tissue squashes
Autor/es:
F. BRAVO-ALMONACID, L. HAIM, Y A.N. MENTABERRY.
Revista:
PLANT DISEASE
Referencias:
Año: 1992 vol. 76 p. 574 - 578
ISSN:
0191-2917
Resumen:
Squash-blot immunoassay was employed to detect potato viruses X and Y in samples obtained from potato leaves and tubers. Comparative assays were performed by both enzyme-linked immunosorbent assay (ELISA) and squash-blot molecular hybridization. Percentages of agreement between these three methods in leaf samples were 95.0% for potato virus X and 97.2% for potato virus Y. About 0.25 ng of virus per 10 mg of leaf tissue could be detected. The method is comparable with ELISA in terms of simplicity, rapidity, and cost. The procedure was also used to analyze virus distribution in different potato tissues.
