Protumoral properties of neutrophil extracellular traps (Nets) in chronic lymphocytic leukemia

PODAZA E.; RISNIK D; SABBIONE F; COLADO A; ELIAS E; ALMEJUN B; BEZARES RF; FERNANDEZ GRECCO H.; BORGE M; TREVANI AS; GIORDANO M

Mar del Plata

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Inmunología; 2016

We previously reported that PMN from CLL patients are prone to release extracellular traps (NETs) in response to PMA. In the present work we extended these findings using ionomycin (1µg/ml) or TNF-α (10 ng/ml) plus LPS (100 ng/ml) to induce NETosis. We found higher concentrations of DNA and increased elastase activity in supernatants from CLL-PMN compared to those from healthy donors (HD) PMN (n=6, p