Opposite effects of amines on lymphocyte- and monocyte-mediated ADCC

SEREBRINSKY G; PALERMO M; GEFFNER J; GIORDANO M; ISTURIZ, MA

INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY

Elsevier

Año: 1988

0192-0561

The effect of different amines on antibody-dependent cellular cytotoxicity (ADCC) activity ofhuman mononuclear cells was tested. Whereas monocyte cytotoxic capacity was significantly stimulated inthe presence of methylamine (MA), dansylcadaverine (DC) and glycine ethylester (GEE), lymphocyte ADCCwas markedly suppressed by these agents. The pharmacological actions of these compounds in our system arenot related to their ability to inhibit transglutaminase (TGase) enzymes, since tertiary amines such assarcosine ethylester (SEE) and chloroquine (CQ) elicited identical responses to MA, DC and GEE. Thecalmodulin (CAM) inhibitors trifluoperazine (TFP) and the more specific N- (6-aminohexyl)-5-chloro-1-naphtalene sulfonamide (W-7) [Hidaka, Sasaki, Tanaka, Endo, Ohno, Fujii & Nagata (1981) Proc. natn.Acad. Sci. U.S.A., 78, 4353 - 4357] mimicked the effects of amines on ADCC, suggesting the possibility thata CAM-regulated process might be involved in the functional changes provoked by amines on ADCC.Finally, binding of ~:~I-immune complexes to the effector cells in the presence of amines showed lack ofcorrelation between alterations in ADCC capacity and FcyR expression.