Cyclophosphamide modulates arachidonic acid metabolism by peritoneal macrophages

M GIORDANO; GEFFNER JR; SEREBRINSKY G; PALERMO M; ISTURIZ, MA

INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY

Elsevier

Año: 1988 vol. 10 p. 939 - 944

0192-0561

The treatment of mice with a single dose of cyclophosphamide (Cy) (200 mg/kg) enhanced thechemiluminescence (CL) response of peritoneal macrophages (PM) triggered with opsonized zymosan (OpZ).The enhanced CL response could not be attributed to the stimulation of the cyanide-insensitive respiratoryburst, since neither superoxide anion release nor immune complex-triggered cytotoxicity, an oxygendependentlytic mechanism, were increased in Cy-PM. Then, products of the oxidative metabolism ofarachidonic acid were measured. It was found that Cy-PM exhibited increased release of prostaglandin E2and leukotriene C4 in response to OpZ when compared with resident PM. In contrast, similar levels ofthromboxane B2 production were observed in both populations.The activation of macrophage arachidonic acid metabolism reported here may contribute to theimmunomodulating action of Cy.