REGULATION OF PH IN CYSTIC FIBROSIS CELLS BY EGFR

MASSIP COPIZ MARÍA M.; VALDIVIESO ANGEL GABRIEL; CLAUZURE, MARIÁNGELES; MORI, CONSUELO; ASENSIO, CRISTIAN; SANTA COLOMA TOMAS A.

Congreso; LXV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2020

SAIC

Cystic fibrosis (CF) is a genetic disease caused by mutations in the CFTR (CF transmembrane conductance regulator) gene. The pulmonary damage observed in severe patients is the major cause of morbidity and mortality due to chronic infections and unresolved inflammation. The reduction in the airway surface liquid (ASL) pH is one of the hypotheses that tried to explain the high susceptibility to lung infections. Together with a reduced bicarbonate transport through CFTR, an increase in lactic acid secretion could also explain the changes in extracellular pH. The aim of the present work was to determine if the EGFR pathway is involved in the pH regulation in CF cells. Two cellular models were used: IB3-1 cells (bronchial epithelial cells derived from a CF patient with a ΔF508/W1282X CFTR genotype) and C38 cells (IB3-1 “corrected” cells). The results reported a decrease in pH in the extracellular medium culture (p