Oligobodies: Bench Made Synthetic Antibodies

RADRIZZANI M; BROCCARDO M.; GONZÁLEZ SOLVEYRA C; BIANCHINI M; REYES, G.B.; CAFFERATA, E.G.; SANTA COLOMA TA,

MEDICINA (BUENOS AIRES)

MEDICINA (BUENOS AIRES)

Lugar: Buenos Aires; Año: 1999 vol. 59 p. 753 - 758

0025-7680

Using synthetic peptides and a combinatorial library of 56 mer random oligonucleotides, we have developed reagents that behave as ?synthetic antibodies?. The results obtained with the protein phosphatase 2A as a model system are shown here. The specificity of these reagents, named ?oligobodies?, has been demonstrated by Western blot analysis and immunohistochemistry. The oligobodies have enormous advantages compared to antibodies: their production is independent of the immune system, they can be prepared in a few days and there is no need for a purified target protein. These reagents can be produced even if the corresponding protein was never isolated or purified, since only a partial DNA sequence from a database provides enough information to make them.