INVESTIGADORES
SANTA COLOMA Tomas Antonio
artículos
Título:
Sex steroid binding protein from Bufo arenarum: Further characterization
Autor/es:
SANTA-COLOMA TA; MUSCHIETTI JP; CHARREAU EH
Revista:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. A: COMPARATIVE PHYSIOLOGY.
Editorial:
Instituto de Biología y Medicina
Referencias:
Año: 1986 vol. 85 p. 401 - 405
ISSN:
0300-9629
Resumen:
The effects of temperature, pH, divalent cations, 2-mercaptoethanol (Et-SH), N-ethylmaleimide (NEM), and phenylmethylsulfonyl fluoride (PMSF) on the dihydrotestosterone (DHT) binding to sex steroid binding protein from Bufo arenarum (baSBP) were examined. The temperature curve indicated that the binding remained stable up to 50 degrees C and the pH curve showed maximum binding between pH 7 and 9. The incubations of baSBP with divalent cations, NEM and Et-SH demonstrated that baSBP require disulfides and sulfhydryl groups for steroid binding or to maintain an adequate protein conformation. On the other hand, PMSF had no effect on the binding, consequently, serine residues appear not to be involved in DHT binding to baSBP. These results indicate that baSBP has a behavior resembling that of human SBP.
