INVESTIGADORES
BUZALEH Ana Maria
artículos
Título:
Hepatic damage and oxidative stress induced by Griseofulvin in mice
Autor/es:
MARTINEZ MARIA DEL CARMEN; AFONSO, SUSANA; MEISS, ROBERTO; BUZALEH, ANA MARIA; BATLLE, ALCIRA
Revista:
CELLULAR AND MOLECULAR BIOLOGY
Editorial:
C M B ASSOC
Referencias:
Lugar: Noisy Le Grand; Año: 2009 vol. 55 p. 127 - 139
ISSN:
0145-5680
Resumen:
Erythropoietic Protoporphyria (EPP) is a disease associated with ferrochelatase deficiency, which produces
accumulation of protoporphyrin IX (PROTO IX) in erythrocytes, liver and skin. In some cases, a severe hepatic failure and
cholestasis was observed. Griseofulvin (Gris) develops an experimental EPP with hepatic manifestations in animals. The aim
of this work was to further characterize this model studying its effect on different metabolisms in mice Gris feeding (0-2.5%,
7 and 14 days). PROTO IX accumulation in liver, blood and feces, induction of ALA-S activity, and a low rate of Holo/Apo
tryptophan pyrrolase activity was produced, indicating a reduction of free heme pool. The progressive liver injury was
reflected by the aspect and the enlargement of liver and the induction of hepatic damage. Liver redox balance was altered due
to porphyrin high concentrations; as a consequence, the antioxidant defense system was disrupted. Heme oxygenase was also
induced, however, at higher concentrations of antifungal, the free heme pool would be so depleted that this enzyme would
not be necessary. In conclusion, our model of Protoporphyria produced liver alterations similar to those found in EPP
patients.