INVESTIGADORES
BUZALEH Ana Maria
artículos
Título:
Relevance of P-450 levels in the actions of enflurane and isoflurane in mice. Studies on the haem pathway
Autor/es:
BUZALEH ANA MARIA; MARTINEZ MARIA DEL CARMEN; BATLLE, ALCIRA
Revista:
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
Editorial:
Blackwell Synergy
Referencias:
Año: 2000 vol. 27 p. 796 - 800
ISSN:
0305-1870
Resumen:
1. The effect of the fluorinated ether anaesthetics enflurane
and isoflurane in mice on haem metabolism and regulation in
different metabolic states, such as depression and induction of
cytochrome P450 produced by allylisopropylacetamide (AIA)
and imidazole, respectively, was investigated.
2. Mice previously treated with AIA (350 mg/kg, i.p.) or
imidazole (400 mg/kg, i.p.) received a single dose (1 mL/kg, i.p.)
of enflurane or isoflurane and were killed 20 min after anaesthetic
administration.
3. Induction of d-aminolevulinic acid synthetase (ALA-S)
activity was found, as expected, in animals receiving AIA and
also in animals treated with AIAplus anaesthesia, but no change
in the activity of either porphobilinogenase (PBGase) or
porphobilinogen deaminase (PBG-D) activities was detected in
these two groups of animals. An additional increase in haem
destruction was observed in the AIA plus isoflurane-treated
group. When mice were injected with imidazol alone or in
combination with the anaesthetics, ALA-S activity was increased
5090% in all groups, but again no change in PBGase or
PBG-D activity was observed. Haem oxygenase was diminished
in mice receiving imidazole and anaesthesia.
4. In conclusion, neither enflurane nor isoflurane caused
additional disturbances in haem metabolism to those produced
by AIA or imidazole alone.