A High Throughput approach to delineate protein complexes in Formalin- Fixed Paraffin-Embedded Prostate Adenocarcinoma and Benign Prostatic Hyperplasia tissues

BIZZOTO, JUAN; LAGE VICKERS, SOFIA; VALACCO PIA; SANCHIS, PABLO A; MAZZA OSVALDO; SCORTICATI CARLOS; NEMIROVSKY SERGIO; COTIGNOLA JAVIER; ELBA VAZQUEZ; GUERON GERALDINE

Congreso; Reunión Anual de la Sociedad Argentina de Investigación Clínica 2019; 2019

Introduction: Prostate cancer (PCa) is a progressive disease involving multiple gene alterations;however, little is known at the proteome level. Most of the functional information of the cancerassociated genes relies in the proteome, a complex biological system with dynamic interactions. Wecarried out an in-depth proteomics analysis to identify differential protein complexes associated withPCa.Methods: PCa and Benign Prostatic Hyperplasia (BPH) samples were obtained from the Hospital deClínicas ?José de San Martín? (with written informed consent and institutional review board approval).Samples were subjected to mass spectrometry analysis (ESI-MS/MS). To identify protein complexesdifferentially expressed we used CORUM, a database of human protein complexes.Results: We identified the following differentially expressed protein complexes in PCa compared withBPH (P