Proteomic characterization of the secretome from prostate cancer and bone progenitor cell co-culture.

ANSELMINO, NICOLÁS; PAEZ, ALEJANDRA V.; LAGE VICKERS, SOFIA; COTIGNOLA, JAVIER HERNÁN; VALACCO, PIA; GUERON GERALDINE; VAZQUEZ, ELBA SUSANA

Orlando

Congreso; Prostate Cancer: Advances in Basic, Translational, and Clinical Research; 2017

AACR

Introduction: Prostate cancer (PCa) is one of the most frequent sites of primary tumor development in human beings. PCa tumor cells tend to disseminate to and form metastatic lesions in the bone. Given the evident prevalence and clinical significance of bone metastases, unraveling the mechanisms exploited by cancer to form these destructive lesions is of critical interest. This is partly due to the fact that the metastatic cascade encompasses many rate limiting steps demanding tumor cells to employ new mechanisms at each stage. Our work aimed at evaluating which soluble factors could mediate the chemical cross-talk between PCa cells and bone progenitor cells.Methods: We used co-culture transwell systems of PC3 (human PCa cells) with the pre-osteoclastic Raw264.7 or the pre-osteoblastic MC3T3 cell lines (murine cells), where cells shared the culture media without direct physical contact. We employed mass spectrometry -nanoLC-MS/MS(Orbitrap)- for the secretome analysis of the conditioned medium (CM). The obtained spectra were analyzed with the Proteome Discover Software and compared with human protein databases. Results highlight a differential profile of proteins released to the CM when tumor cells are grown in co-culture, compared to controls.