Local effects of low doses of PTH 1-34 on experimental periodontitis

DAVISON MR, ; BIDEVICH NL, A; PRELIASCO M, ; BONANNO MS, ; ZENI SN.

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Congreso; . Reunión Anual de Sociedades de Biociencias (SAIC-SAI-SAFIS). Abstract; 2020

SAIC

Periodontitis is a chronic disease with high prevalence in adults that induces a progressive alveolar bone loss leading to tooth loss. Intermittent PTH has anabolic and anti-inflammatory effects, two properties necessary to avoid the side effects of periodontitis. Experimental periodontitis can be induced in rats in few days, via cotton ligatures placement in the gingival sulcus around the molar teeth. The biofilm accumulation and disruption of the gingival epithelium induce bone resorption conducting to bone loss. We investigated whether intermittent administration of a low dose of PTH 1-34 would reduce alveolar bone loss in the experimental periodontitis induced in 16 female Wistar rats (221±15g) under light anesthesia. Ligature was replaced every 4 days. Rats were randomly divided in two groups which where subcutaneously injected with: G1 saline solution and G2 1.2ug PTH1-34. every 48 hs. for 18 ds. Eight rats were left unligated as healthy control. At the end of the study blood was obtained and CTX was evaluated. After sacrifice, hemimandibles were extracted and fixed in formalin buffer for histologic analysis of tibia subchondral bone volume (BV/TV%), alveolar bone BV/TV% and periodontal space height (PSH). Results: tibia BV/TV%: C 38.77±2.59; G1 38.29±3.9 and G2 37.75±1.45; alveolar bone BV/TV% C 50.3±3.6c; G1 35.6±4.3a and G2 42.0±1.4b; PSH (mm): C: 0.196±0.057a; G1 0.809±0.115b and G2: 0.706±0.065c.. CTX (pg/mL): C 49.3±7.8b; G1 65.8±7.6a and G2 439±3.7b. Different letters show a p