EFFECTS OF THE ADDITION OF TERIPARATIDE TO A BOVINE BONE GRAFTING MATERIAL ON THE REPAIR OF CRITICAL-SIZED BONE DEFECTS IN RATS. PRELIMINARY RESULTS

PELEGRINI G;; BONNANO M; ZENI CORONEL EM; ORZUZA R; AVENDAÑO ME,; ZENI SN

Congreso; REUNIÓN CONJUNTA SAIC SAI&FAIC SAFIS 2022; 2022

We previously determined the osteoconductive effect of a bovinebone graft manufactured in Argentina [Synergy Bone Matrix, OdontitImplant Systems, Argentina (SBM)], on the bone healing processin an experimental model in rats. Several studies have shown thatintermittently administered PTH has an anabolic effect on cancel-lous and cortical bone. On these bases, we hypothesized that theaddition of the synthetic PTH analog, Teriparatide (TPT), to SBMcould accelerate bone regeneration. The aim of the present experi-mental study was to evaluate if the addition of TPT to SBM presentsany additional beneficial effect versus the use of SBM alone. Thirtyadult male Wistar rats of 333±39.22 (n=6/group) and a critical-sizebone defect (CSD) was created in the medial aspect of both tibiae.All CSDs received one of the following treatments for 30 days: G1:control without treatment; G2: SBM (Lot No: E11121216); G3: SBMmixed with 20 μg of T (Osteofortil, BioSidus, Argentina); G4: injec-tion of 0.125 μg/day of TPT and G5: SBM mixed with 20 μg of TPTand SC injection of 0.125 μg/day of the same drug. The animalswere sacrificed 45 days after the beginning of the experiment. Weanalyzed serum PTH, P1NP and CTX (ELISA). At the end of thestudy, the tibiae were evaluated with X-ray microtomography (μCT).Serum PTH levels (pg/mL) at the end of the study were 4.6±1.2a;11.3±2.3b; 16.4±0.7c; 17.9±0.8c and 19.1±0.5c, respectively, P1NPlevels (pg/L) at the end of the study were 2.24±0.24a; 2.63±0.16c;2.54±0.37c; 2.43±0.41b; 2.48±0.45b; respectively. Preliminary μCTresults showed: G1 no bone defect repair; G2: poor repair; G3: re-pair; G4: overgrowth of bone tissue around the bone callus and G5:repair without bone overgrowth. Our findings showed that the ad-dition of TPT to SBM mixture accelerated the bone repair processwhile the daily injection did not produce any additional improvementin the bone regeneration exerted by SBM.